With regard to samples A-M, the real-time PCR analysis reveals a very low initial amount of DNA, with the maximum measurable DNA concentration at 0.005 ng/μL (5 pg/μL) in sample “I”. The following table reports the measurements relative to the indiviudal samples examined in triplicate. It should be noted that the analysis conducted on the internal control (IPC) does not reveal anything suggestive of an inhibition of the PCR reaction. In fact, the value of Ct relative to the IPC turns out to always lie in the interval between 28-31, in line with expectation. In other words, from the analysis performed one can rule out the presence of inhibitors of the PCR reaction capable of compromising the DNA amplification in the individual samples analyzed. The concentration of DNA in the majority of the samples examined turned out to be undeterminable (Undetermined).
Finally, for each DNA sample (A, B, C, D, E, F, G, H, I, L, M, CN, and NTC), we report below the plot [tracciato] relative to the PCR reaction. Each plot (Male SRY, Human RPPH1 and IPC) consists of three curves (triplicate), each of which is shown with three different colors as indicated in the tables recorded for each plot:
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